An oligo file contains primer set or probe information saved within the
OligoCheck program from the main window file menu. This is a simple
text file that can be opened and edited within any text editor program
(although there shouldn't be any need to do this!).
When creating an oligo file, make sure that the primers or probes being
saved contain only recognised IUB base
codes, otherwise an error message will be displayed.
Oligonucleotides should always be sense
and written from 5' to 3'. Antisense oligonucleotides can be
converted with the aid of ReverseComplement
if necessary. 3'
position information relative to the
Escherichia coli 16S rRNA gene can
be supplied, although this is not mandatory.
Contents of a typical oligo file (formatted and coloured to make it
easier to read):
<?xml version="1.0"
encoding="UTF-8"?> <!--File
generated by OligoCheck. Copyright (c) 2004, Kevin Ashelford.--> <oligos>