AY354817 report

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AY354817 Report

AY354817 is responsible for 7 of the outlier DE values.
AY354817 is confirmed as anomalous by following the recommended anomaly confirmation protocol.
Further checking to confirm chimeric status shows AY354817 to be a two fragment chimera, with its 5' end most similar to the Alphaproteobacteria sequence AY697909 (Fig. 1a) and its 3' end most similar to the Bacteriodetes sequence AY344418 (Fig. 1b).
Breakpoint approximately at base position 600.

Figure 1a. Variation in % difference between AY354817 (1013 nt) and AY697909 (1425 nt), determined with a 300 base window, moving 25 bases at a time along the sequences' length. Profile is characteristic of a two fragment chimera, with the break-point occurring at roughly base position 600.

Figure 1b. Variation in % difference between AY354817 (1013 nt) and AY344418 (1493 nt), determined with a 300 base window, moving 25 bases at a time along the sequences' length.