AY354824 report

www.cardiff.ac.uk/biosi/research/biosoft/

AY354824 Report

AY354824 is responsible for 10 of the outlier DE values.
AY354824 is confirmed as anomalous by following the recommended anomaly confirmation protocol.
Further checking to confirm chimeric status shows AY354824 to be a two fragment chimera, with its 5' end most similar to the Alphaproteobacteria sequence AY697909 (Fig. 1a) and its 3' end most similar to the Alphaproteobacteria sequence AY033327 (Fig. 1b),
Break point around E. coli position 200.

Figure 1a. Variation in % difference between AY354824 (1055 nt) and subject AY697909 (1425 nt), determined with a 100 base window, moving 25 bases at a time along the sequences' length.

Figure 1b. Variation in % difference between AY354824 (1055 nt) and AY033327 (1121 nt), determined with a 100 base window, moving 25 bases at a time along the sequences' length.