Bioinformatics toolkit
www.cardiff.ac.uk/biosi/research/biosoft/

Mallard Check List


Before starting an analysis, check the following for a reliable, error free analysis:
  1. Do all sequences share the same (sense) orientation?
  2. Do all sequences cover roughly the same region of the 16S rRNA gene sequence?
  3. Do all sequences contain only valid IUB base characters?
  4. Does your input file include a suitable reference sequence?
  5. Are the sequences in your input file aligned and in the correct format?

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Dr K.E. Ashelford. © 2006, Cardiff University