A simple tool for checking and altering the orientation of 16S rRNA gene sequences.
To download OrientationChecker, click on the button below. Alternatively visit this site's download page.
Please make sure you have the Java Runtime Environment installed on your computer.
KE Ashelford et al. (2006) "New Screening software shows that most recent large 16S rRNA gene clone libraries contain chimeras." Applied and Environmental Microbiology, 72(9): 5734-5741.
OrientationChecker is a simple tool for quickly locating the position of partial sequences within the 16S rRNA gene, and determining (and, if necessary, altering) their orientation, without the need for time-consuming multiple sequence alignments.
This is open source software and is released under the terms of the GNU General Public Licence.
This program is written in Java, and will therefore run on any operating system capable of supporting this runtime environment. Java is available on Microsoft Windows, Apple Mac OS X, and other Unix platforms including Linux. Please refer to this page for further details. Note: this program requires Java version 1.4.x or later.
For further information, click here.
This software was designed and written by Dr Kevin Ashelford. Any comments, suggestions or bug reports - please contact me at ashelford@cardiff.ac.uk.
This work has been supported by the Biotechnology and Biological Sciences Research Council (BBSRC).